ISFET based biosensor for heavy metal ions
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Abstract
In project work, we studied the effect of heavy metal ions on enzymatic activity of
free and encapsulated whole cells using ISFET after optimizing the concentration
of biomaterial, substrate and incubation time for desired changes in potential. In
presence of heavy metal ions activity of enzymes (alkaline phosphatase) present in
whole cell membrane decreased causing the change in potential measured with
ISFET. All metal ions showed same effect on enzymatic activity but to different
extent as given below:
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Master of Science-Chemistry, Dissertation