Purification and stability analysis of S. cumini anthocyanins

Abstract

Syzygium cumini, commonly known as jambolana or Indian blackberry, is a fruit rich in anthocyanins with significant health-promoting properties. This study used conventional solvent extraction (using methanol as solvent) to recover anthocyanin from Syzygium cumini pulp. The TAC (Total anthocyanin content) was calculated and measured using the spectrophotometer which allowed rapid and accurate estimation of anthocyanin concentration. Additionally, the purification methods of S. cumini anthocyanins were investigated using two different methods: ATPS (Aqueous two-phase separation) and column chromatography. Regarding the Stability analysis, three parameters were taken into consideration; Light, pH and temperature. Each parameter was studied by subjecting purified samples to different incubation conditions and results were observed accordingly.