Studies of Glucose Oxidase Immobilized Carbon Nanotube - Polyaniline Composites

Abstract

ABSTRACT Glucose biosensor is an analytical device that responds in a direct reversible, continuous, rapid, and accurate manner to changes in the concentration of chemical or biochemical species in an untreated sample. It consists of a sensing microzone where a chemical or biochemical reaction takes place. This is connected or integrated with a transducer. The performance and usefulness of these types of biosensors are often dictated by the immobilization methods and the immobilization matrixes employed for the deposition of the enzyme layer. For example, the sensor lifetime, its dynamic range, sensitivity, selectivity, response time, and stability are some of the operational parameters affected by the enzyme immobilization procedure and the type of support materials used for the biosensor fabrication. The aim of this thesis was to study the I-V Characteristic of Glucose Oxidase Immobilized Carbon Nanotube-Polyaniline Composites. MWNTs were functionalized and enzyme is immobilized on the acylated MWNTs. The f-MWNTs and GOD-MWNTs were characterized by FT-IR and FE-SEM. Polyaniline, MWNT-PANI, c-MWNT-PANI, a-MWNT-PANI composites were synthesized by in situ chemical polymerization process and enzyme was immobilized on the c-MWNT-PANI and a-MWNT-PANI composites. These composites were characterized by FT-IR and FE-SEM. The films of these synthesized materials were prepared by solution casting method on the glass substrate and I-V characteristic were studied. The amperometric response of GOD-MWNTs film was studied at constant voltage (Real Time measurement) and variable voltage sweep. The current was found to be increased when glucose solution was dropped on the sensing zone of the film. The result shows the successful synthesis of glucose sensing material.