Simultaneous Saccharification and Fermentation of Bioethanol From Starchy Based Kitchen Food Waste

Abstract

Ethanol is one of the renewable energy sources with high potential and low environmental impact. Various raw materials have been used as for ethanol production. In this study, waste potato mash was chosen for ethanol production; however, a pretreatment process named as Liquefaction and Saccharification is needed to convert starch of potato to fermentable sugar directly by using microorganismsAspergillus oryzae and Saccharomyces cerevisiae instead of using expensive enzyme. This is a novel concept which was proposed by few scientists but much research is done on this subject. So, the process of SIMULTANEOUS SACCHARIFICATION AND FERMENTION directly by microorganisms is being introduced. In order to obtain maximum fermentable sugar conversion, optimum parameters for the liquefaction and saccharification processes were optimized such as temperature,amount of potato mash,pH, agitation rate, length of fermentation. The optimum combination of temperature, and amount of potato mash was determined as 30°C,5% of inoculum size, and 15 g dry-weight potato mash /100 ml DI water. The effect of pH, inoculum size and various sources to obtain maximum ethanol from waste potato mash was under batch fermentation. In conclusion, this study has given the potential for utilization of potato waste for ethanol production.Ethanol is basically produced by fermentation by well known fungal strain Saccharomyces cerevisiae.In our study we have used an economic and sustainable process of Simultaneous Saccharification and Fermentation by the two potent fungal strains Aspergillus oryzae and Saccharomyces cerevisiae.