Phytochemical Analysis and Green Synthesis of ZnO and Ag/ZnO Nanocomposites from Lansium Parasiticum for the Evaluation of their Toxicology

Abstract

The increasing trend in the popularity of green synthesis owing to its cost effective and ecofriendly attributes, the unscathed plant Lansium parasiticum is evaluated for its phytochemical makeup and the anthelmintic activity of its extracts in various solvents. Methanolic and Aqueous plant extracts proved effective as anthelmintic agents. ZnO nanoparticles were synthesized by direct precipitation method using zinc acetate and sodium carbonate as precursors and biosynthesized using the plant extract. The Ag doped ZnO particles were also synthesized in various concentrations in a green route of synthesis. The synthesized particles were characterized by UV, XRD, SEM and DLS.The X-Ray diffraction studies reveal that both the chemically and biosynthesized ZnO have a crystalline monoclinic structure. The UV-Visible spectra shows the ZnO peak at 284 nm in case of green ZnO and the shift in the absorption band in case of different concentrations of Ag-ZnO nanocomposites. DLS studies show that the average particle size of the biosynthesized ZnO without an external capping agentwas 210 nm and that of the different concentrations of Ag-ZnO nanocomposites ranged from 45-210 nm. After the addition of an external capping agent it was reduced to 19- 30 nm. The as-synthesized ZnO and Ag-ZnO nanocomposites were also tested for their antibacterial activity against the facultative pathogenic bacteria- E.coli. Colony counting and broth dilution methods were used. In both the methods 200 µg/ml was found out to be the MIC.