Screening of Endophytic Fungi for the Inhibition Of Indolamine 2,3 Dioxygenase

Abstract

Indolamine 2,3 dioxygenase (IDO) found inside the human body which is responsible for causing various chronic diseases such as Alzheimer, Parkinson, cancer etc. Endophytic fungi are microorganisms which resides inside the plant tissue which contains huge bioactive compound such as Taxol and is known as gold mines of enzyme inhibitor. Various enzyme inhibitor has been isolated and reported from endophytic fungi such as huperzine, Lovastatin, Peptide etc. Current studies deals with the production of bioactive compounds from endophytic fungi for the inhibition of IDO enzyme. The present approach deals with isolation and screening of endophytic fungi for the inhibition of IDO. 26 endophytic cultures were isolated from small segments of apple (M. pumila) plant. Out of which 10 fungal extract exhibited above 70% inhibition during preliminary test. Formation of intense yellow colour shows the presence of high kynurenine production with high OD value which signifies presence of poor inhibitor or low kynurenine production with low OD value which signifies presence of strong inhibitor at 492nm. Among them #77MPLSH fungal extract resulted above 80 % inhibition of IDO. Therefore, On the basis of preliminary data, #77MPLSH was found to be potent inhibitor of IDO. So, further molecular identification, extraction and morphological studies were done and the potential culture #77MPLSH was tentatively identified as Botryosphaeria sp. Hence, due to strongest inhibition activity against IDO, it was concluded that #77MPLSH could be considered as a potential inhibitor from endophytic fungi and further studies can be proceeded to isolate novel compounds