SNP genotyping of reck gene in lung cancer

Abstract

RECK is reversion inducing cysteine rich protein with Kazal motifs that inhibits matrix- metalloproteinases such as MMP-2, MMP-9 and MT-1 which in turn cause breakdown of extracellular matrix and also inhibits angiogenesis. For SNP genotyping of RECK gene, a total of 100 lung cancer samples and controls were analyzed for RECK SNPs (rs11788747) using PCR- RFLP genotyping analysis. After adjusting the co-variants, the individuals carrying RECK promoter (rs11788747) inheriting at least one G allele had higher risk of developing lung cancer as compared to wild type carriers. Individuals carrying heterozygous genotype (A/G) had significant correlation with developing of lung cancer (OR 0.398, 95% CI; 0.24-0.775, p = 0.006). Individuals with heterozygous (A/G) genotype had significant correlation with smoking in smokers (OR = 0.338, 95% CI; 0.141-0.814, p = 0.014) and But non smokers those who having mutant genotype (G/G) had 3 times more risk of lung cancer (OR = 3.000, 95% CI; 0.157-57.365, p = 0.45). RECK gene polymorphism might be a risk factor in increasing lung cancer susceptibility.