Synthesis of Bis-coumarin Based Probe for the Detection of Serum Albumin

Abstract

Bis-coumarin based probe has been synthesized which provides insight into the differential response on interaction with different bioanalytes. Photophysical studies has been performed to detect the bioanalytes and it has been observed that probe 5 selectively detects the serum albumin i.e., BSA and HSA with more sensitivity towards HSA. On gradual addition of HSA (62 M), 74% quenching has been obtained while in case of BSA (62 M) 60% quenching was observed. Further, binding constants have been calculated to demonstrate the effective binding between the synthesized probe 5 and the serum albumins and found to be 7.4 x 105 M' in case of HSA while 6.7 × 105 M' for BSA. Moreover, site marker study has also been carried out to find out the specific binding site of the probe 5 in HSA which revealed the warfarin like binding of the probe 5 i.e., Suldow's site I.