Identification of Peptide Containing T Cell Epitope Of Hemagglutinin Protein In H5n1 Influenza Virus

Abstract

HPAI A (H5N1) highly pathogenic avian influenza virus of type A of subtype H5N1 is a highly pathogenic causative of Avian flu in human. Conserved regions from Hemagglutinin (HA) of H5N1 of influenza virus A strains isolated from human hosts were identified (≥ 80% conservation) by computational tool based on informational entropy. Epitopes for HLA alleles of MHC class I and class II from the conserved regions were predicted using three different epitope prediction tools separately for MHC class I and II. Predicted epitopes were screened for the presence of any similarity with human self-proteins. 27 MHC class I and 27 MHC class II epitope were identified. Overlapping epitopes were merged to generate putative immunogenic peptide containing both MHC class I and Class II predicted epitopes. Seven such sequences were identified, having minimum conservation 81%. One of the sequences RRIENLNKKMEDGFLDVWTYNAELLVLM (CS14) has shown 100% conservation followed by NSSMPFHNIHPLTI (CS12) which was 98.92% conserved. All these sequence can be considered as the potent candidates for vaccine design against H5N1.