Effect of Exopolysaccharides Produced under Stress Conditions by Dunaliella Salina on the Growth of Cancer Cell Lines and Peripheral Blood Mononuclear Cells

Abstract

Dunaliella species is well known to produce various bioactive metabolites such as carotenoids (β-carotenoid, phytoene, phytofluene, lutein, and zeaxanthin), dried algal meal & glycerol that have been commercialized due to their antioxidant, anti-cancerous properties. In the current study, the cell growth effect of exopolysaccharide (EPS) produced under stress conditions were assessed in peripheral blood mononuclear cells (PBMC).Glucose content was found to be increased in stress conditions as compared to normal. EPS was found to be increased in stress conditons Cell growth promoting effect was observed in PBMC by EPS produced and the effect was higher in stress than normal conditions. Isolated EPS from D.salinawith different stress conditions i.e. NaCl 3.5 M, KNO3 (3X), KNO3 (5X), KH2PO4 (5X) showed highest inhibition as compared to normal on RAW 264.7 cells. However KH2PO4(3X) showed highest inhibition as compared to normal on MCF-7 cells.The FTIR data confirmed the presence of alcohol, keto group, nitro group and halo functional groups.1H NMR data confirmed the presence of hexose & pentose sugar with their particular chemical shifts. The obtained spectra showed hexose sugar, trisaccharides, and pentasaccharide. Hence it is concluded that enhanced glucose content in EPS produced under stress condition may be associated with increase bioactive properties.