Isolation and Characterization of Xanthine Oxidase Inhibitor from Endophytic Fungi of Aegle marmelos

Abstract

Hyperuricemia is initiated by the overproduction or underexcretion of uric acid in the body ultimately leading to gout. The key enzyme responsible for this pre-disposing factor is xanthine oxidase (XO) which oxidises xanthine to uric acid. The work presented here aims to isolate the xanthine oxidase inhibitors of the pre-screened endophytic fungi isolated from <i>Aegle marmelos<i>. The fungus extract after evaluation of XO inhibition activity was subjected to coloumn chromatography for isolation of purified compounds. All the fractions obtained were subjected to qualitative NBT test for maximum inhibition towards XO. Fraction 1 which is having the least polarity showed best results that was then subjected to antioxidant and antimicrobial assay. The fraction was subjected to different phytochemical tests for the presence of various secondary metabolites. Genomic DNA of the fungus was isolated and amplified using PCR. The pure fraction was then subjected to different spectroscopic techniques for characterizing the compound.