Macromolecular Crowding-Induced Molten Globule State of Acid-Denatured Horse Cytochrome c
| dc.contributor.author | Goyal, Neha | |
| dc.contributor.supervisor | Kumar, Rajesh | |
| dc.date.accessioned | 2014-09-04T06:57:26Z | |
| dc.date.available | 2014-09-04T06:57:26Z | |
| dc.date.issued | 2014-09-04T06:57:26Z | |
| dc.description | MS, SCBC | en |
| dc.description.abstract | This work examined the effect of concentration and size of crowding agents (dextran 40 and dextran 70) on structure, stability and folding of acid-denatured ferricytochrome c at pH 2.0. As [dextran] is increased in the UA state , the intensity of flouroscence emission spectra decreases, which indicates molecular compaction of UA state. The crowding agents stabilize and refold the acid-denatured ferricytochrome c (pH 2.0) to MG-states. The acid-denatured ferricytochrome c also gains substantial secondary structure content but not acquire tertiary structure content in the presence of high concentrations of crowding agent. | en |
| dc.format.extent | 523457 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10266/3147 | |
| dc.language.iso | en | en |
| dc.subject | Molten Globule State | en |
| dc.subject | Cytochrome c | en |
| dc.title | Macromolecular Crowding-Induced Molten Globule State of Acid-Denatured Horse Cytochrome c | en |