Assessment of In Vitro Antimicrobials and Enzyme Inhibitors from Indian Muscodor Species

Abstract

The current study explores endophytic Indian Muscodor isolates for their antimicrobial, enzyme production and α-amylase inhibitory potential. Initially the eight Muscodor sp. were subjected for production of cell free culture broths via shake flask method. These culture filtrates were further tested for their antimicrobial potential against a battery of gram positive and gram negative bacteria. Out of all the eight screened Muscodor isolates, ethyl acetate fraction of #130 TMDSTYEL exhibited maximum antimicrobial activity whose zone of inhibition ranges from 8-17 mm in agar well diffusion assay followed by #6(B) CCSTITD whose zone diameter ranges from 9-14 mm. Minimal Inhibitory Concentration (MIC) of #130 TMDSTYEL ranges from 62.5-250 μg/ml where as its IC50 was found to be ranging between 0.125-2 mg/ml against the tested spectrum of microorganisms. These Muscodor isolates were then screened for production of industrially important enzymes like cellulases and amylases via plate assays. #130 TMDSYEL and #6(B) CCSTITD were found to produce α-amylase whereas #1 CCSTITD, #6610 CZSTITD and #2 CCSTITD produced cellulases respectively. The Muscodor isolates were also screened for their α-amylase inhibitory potential where #6(B) CCSTITD, #130 TMDSYEL and #16AMLWLS induces inhibition of α-amylase enzyme by 99.19%, 98.59% and 73.46% respectively. The current study is the first attempt to harness the secondary metabolites (apart from volatile organic compounds) produced by Muscodor species for biological activities like antimicrobial and enzyme inhibitors. Thus from the above obtained results it can be concluded that Indian Muscodor species are prolific producers of biologically active molecules which can be further exploited in pharmaceutical and agro industries.