Activity of the Immobilized Candida Rugosa Lipase
| dc.contributor.author | Singh, Kavya | |
| dc.contributor.supervisor | Ali, Amjad | |
| dc.date.accessioned | 2012-09-18T09:31:07Z | |
| dc.date.available | 2012-09-18T09:31:07Z | |
| dc.date.issued | 2012-09-18T09:31:07Z | |
| dc.description | Master of Science (Chemistry) | en |
| dc.description.abstract | In the present study Candida rugosa lipase has been immobilized on MCM- 41 support through sol – gel process. The immobilized enzyme was entrapped by the poly condensation of silica precursors namely tetramethoxyorthosilane /tetraethoxyorthosilane(TEOS) and iso-butyltrimethoxysilane (iso- BTMS). The immobilized enzyme was characterized by scanning electron microscopy, Fourier Transformed Infrared (FT–IR) and UV–Visible Spectrophotometer. Kinetic parameters viz., Michaelis-Menten constant (Km), Maximum rate of reaction (Vmax), Turnover number (kcat) and Specificity constant (kcat /Km) for immobilized Candida rugosa lipase were determined by using Michaelis-Menten and Lineweaver- Burk approach. | en |
| dc.description.sponsorship | School of Chemistry and Biochemistry, Thapar University | en |
| dc.format.extent | 1354178 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10266/2046 | |
| dc.language.iso | en | en |
| dc.subject | Immobilization, Lipase | en |
| dc.subject | Candida-Rugosa | en |
| dc.title | Activity of the Immobilized Candida Rugosa Lipase | en |
| dc.type | Thesis | en |