In Vitro Regeneration Studies of Sugarcane

Abstract

Mass propagation protocol, callus induction and subsequent plant regeneration in vitro in Sugarcane cultivars, CoS 8436 and CoJ 83 were worked out. Axillary bud induction observed best (80-95%) from field grown (8-12 months old) shoot tip spindle explants within 7-10 days in semi solid MS media supplemented with BAP (0.5 mg/l) and Kn (0.5mg/l), however maximum shoots/explant (4.25 - 5.75) were observed in MS + BAP (1.5mg/l) + Kn (1.5mg/l). After two cycle serial subculture in semi solid media for establishment, shoot clumps were multiplied in liquid media, provide higher multiplication rate (3.63-4) in MS + BAP (1.0mg/l) + Kn (1.5mg/l) and higher shoot elongation in MS + BAP (0.5mg/l) + Kn (0.5mg/l). In vitro grown shoots/shoot clumps show earlier root induction (5-7 days), higher no of roots and root length in MS+ NAA (5 mg/l) + sugar (70gm/l). Spindle explants show better callus induction response in MS + 2, 4-D (3mg/l) within 14 days in dark and multiplied in reduced/half conc. of auxin. Plantlet regeneration (68-74%) were best obtained from compact nodular, morphogenic calli cultured in MS media supplemented with BAP (0.5mg/l) and Kn (0.5mg/l) cultured in light and show similar responses for shoot multiplication, elongation and rooting.