Preparation, Characterization and Evaluation of Albendazole Loaded Solid Lipid Nanoparticles from Beeswax for Screening of its Anti-Helminthic and Anti-Cancer Properties

Abstract

SLNs are the non-toxic, biodegradable, nanospheres with a solid lipid matrix that are being employed for targeted drug delivery applications. These particles have shown sustained drug release pattern and are known to improve the aqueous solubility of hydrophobic drugs. In the present study, Albendazole drug, known for its poor water solubility, was loaded in SLN particles that were produced using the methanol extract derived from the beeswax and checked for its effects on the helminthic worm Haemonchous contortus and lung cancer cell line A549. In-vitro studies on A549 cell line was done to examine the cytotoxicity of these particles against the cancer cell line and it was found to be more toxic when Albendazole drug was loaded in the particles than in comparison to the SLN particles (without drug). DLS was used to determine the size of these particles and FT-IR and RAMAN spectrum were studied for confirmation of formation of SLN-Abz particles.