Detection of Gluten by Surface Plasmon Resonance Spectroscopy

Abstract

Gluten is detected by Surface Plasmon Resonance Spectroscopy in this thesis. This detection is primarily based on the interaction of protein with silver and gold Nanospheres. Silver nanospheres are prepared by seedless method and gold nanospheres are prepared by seed mediated technique. Bovine Serum Albumin (BSA) is used as standard/test protein. Concentration dependent interaction of BSA and Gluten with silver nanospheres is exponential in nature while gold shows linear dependency for with both the proteins. Moreover, AgNSPs has nearly thousands times lower detection limit for both BSA and Gluten as compared to AuNSPs. Improved plasmonic sensitivity of silver nanospheres as compared to gold nanospheres make it better probe for bimolecular detection of proteins.