Spectroscopic studies on the interaction ofLevofloxacin with Picric acid as an anionic reagent - A Green Approach

Abstract

Levofloxacin, an antimicrobial agent has been determined by using picric acid as an anionic reagent in aqueous medium. On mixing the reactants in distilled water a yellow colored product is separated within two minutes which was found to be an ion-association complex (LFX-PA) of both the reactants. Spectroscopic studies were carried out on the ion-pair complex in different solvent media to explain the interaction between levofloxacin and picric acid. The spectroscopic studies confirmed its formation as determined from substantial shift in respective λmax values of the ion-pair. A study on the solvent effect by spectrophotometric method for LFX suggested that water is the best medium for the study. A Calibration curves were drawn in acetonitrile, water- acetonitrile and acetonitrile media using the absorbance values of the LFX. Based on better performance calibration curve was drawn in aqueous medium. A linear increase in absorbance was observed with increasing LFX concentration with a lower detection limit of 6×10-5 M in aqueous medium. Further, analysis of LFX in commercially available drug tablets proved that the proposed method is valid in real life sample analysis using the green approach.