Green Synthesis of Bimetallic Nanoparticles Using Aqueous Extract of Deparia boryana, a Himalyan Pteridophyte to Develop Novel Antimicrobial Agent

Abstract

Increasing trend in the popularity of green synthesis owing to its cost-effective and eco-friendly attributes, the plant Deparia boryana, a Himalayan pteridophyte is evaluated for its phytochemical makeup and its antibacterial and antihelmintic activities of its various extracts and biosynthesized nanoparticles. The chloroform, petroleum ether and silver nanoparticles were proved to be effective antihelmintic and antibacterial agents (against E.coli and Bacillus subtilis) while other nanoparticles like TiO2 nanoparticles, TiO2 coated Ag nanoparticles and Ag-coated TiO2 nanoparticles were proved to be antibacterial agents only. The MIC was found to be 1mg/ml. All the nanoparticles were synthesized by green synthesis method that is under sunlight without any chemically added reducing or capping agent. The synthesized nanoparticles were characterized by UV-Visible spectroscopy, FTIR, SEM-EDS and DLS. The UV-Visible spectra for AgNP, TiO2 nanoparticles, TiO2 coated AgNP, Ag-coated TiO2 nanoparticles were observed around 400- 412 nm, 280-320 nm, 405-427 and 284-328 nm, respectively. The DLS studies show that the average size of AgNP and TiO2 coated AgNP were found to be around 30-90 nm i.e. within nano range, whereas the average size of the TiO2 nanoparticles and Ag-coated TiO2 nanoparticles were found to be 90-140 nm, i.e. within the micro range.