Class I Patatin Genes from Indian Potato(Solanum tuberosum L.) Cultivars: Molecular Cloning and Partial Characterization

Abstract

Promoter is the region of DNA that initiates and regulates transcription of particular gene. Strong promoter permits a high rate of transcription. So to drive the expression of any gene of interest either in a constitutive or cell-type specific manner a strong promoter is required. In potato tubers, patatin is a major soluble and storage protein. Based on sequence features, patatin gene is further classified into two classes: Class I and Class II. The present study is focused on molecular cloning and characterization of patatin gene promoters from Indian potato cultivars namely Kufri Chipsona-1 (CS-1) and Kufri Jyoti (KJ). Primers were designed based on the class I patatin gene sequence available in GenBank database. Two different forward primers and one reverse primer were used to amplify promoter region of CS-1 and KJ. The PCR amplified DNA products were cloned in pUC19 vector. The partial characterization was done through PCR and restriction digestion. Putative recombinant clones are likely to be class I specific and can be further characterized using sequencing approach. Further characterization is required in order to estimate promoter’s efficiency to derive a foreign gene expression and to have knowledge of gene regulation.