The Role of Gene X Deletion in Enhancing Secondary Metabolite Production in Fungi

Abstract

The production of secondary metabolites in fungi is tightly regulated by various genetic and environmental factors. One of the key regulatory mechanisms is carbon catabolite repression (CCR), mediated by the transcriptional repressor Gene X. This thesis explores the impact of gene X gene deletion on secondary metabolite production in several fungal species. Additionally, the thesis discusses the use of In-Fusion cloning as a technique for constructing gene deletion cassettes, facilitating targeted genetic modifications in fungi. The study combines literature review, experimental data, and comprehensive analysis to provide a detailed understanding of the potential applications of gene X deletion and In-Fusion cloning in fungal biotechnology.