Please use this identifier to cite or link to this item:
Title: MADS-box Transcription Factors in Potato (Solanum tuberosum L.): Molecular Cloning, Sequence Analysis, Comparison, Prediction of the Crucial Motifs and three Dimensional (3-D) Structures
Authors: Kaur, Ravneet
Supervisor: Das, Niranjan
Keywords: Potato (Solanum tuberosum L.);Tuberization;MADS-box transcription factors;POTM-1;cDNA clones
Issue Date: 14-Sep-2022
Publisher: TIET, Patiala
Abstract: In terms of production and consumption rates, potato is the most significant vegetable crop in the world. It is a major contributor to the global food security. It is a significant source of nourishment and income for people of various communities. Agriculture is facing more and more difficulties due to global climate change. Potato plants are extremely susceptible to high temperatures. Tuberization is a key survival mechanism for potato plants. Understanding factors that regulate the process of tuber induction is critical for developing tuber yield and quality improvement methods. MADS-box genes encode transcription factors that play an important role in plant growth and development, particularly in floral organ determination. In eukaryotes, the MADS-box family of transcription factors has been extensively studied for their critical involvement in transcriptional control. A cDNA of potato MADS-box gene (POTM1-1) from superior cultivar has already been identified and described from an early tuber cDNA library, but very little research has been done using Indian potato cultivars. So, in this study Indian potato cultivar was used as a source to isolate and characterize MADS-box gene. RNA was isolated from various tissues of potato plant and RT-PCR was performed using the total RNA. Further molecular cloning was performed using total RNA from tuber only. The cDNA clones obtained were further characterized by restriction digestion and PCR. Then, sequencing of these cDNA was done. The sequence obtained, designated as POTM1-1A, was submitted to GenBank (Accession No. ON804239). This sequence was compared with other MADS-box genes of various plant species on both nucleotide and protein level. Sequence analysis and motif prediction was done using various bioinformatics tools. The 3-D protein structures of our predicted protein and some homologous sequences from other plant species were also predicted in order to understand the structural and functional relationship.
Description: MSc Biotech Dissertation
Appears in Collections:Masters Theses@DBT

Files in This Item:
File Description SizeFormat 
Ravneet Kaur (Reg No 302001012)_MSc Biotech Dissertation.pdfMSc Biotech Dissertation3.28 MBAdobe PDFView/Open    Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.