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|Antioxidant Activity of the Endophytic Fungi Isolated from the Medicinal Plants
|The field of free radicals has been gaining attention, it has been known that free radicals are an important component of our body’s developing system and also holds significance in signal transduction pathways. The source of free radicals is either body’s normal essential metabolism or exogenous sources such as heavy metals, industrial pollutants, tobacco smoke etc. Diseases such as cancer, cardio-vascular and some neurological disorders are called as salient “Free radical diseases”. The main aim of this work was to study antioxidant potential of crude extracts obtained from endophytic fungi which were isolated from two medicinal plants Eucalyptus terreticornis and Tinospora cordifolia. DPPH assay, total phenolic content, total flavanoid content was performed to check for antioxidant potential of the crude fungal extracts. Based upon results, best activity giving fungi was selected and phytochemical analysis, fractionation of extract through TLC was performed and the activity of the extracted compound was also checked. GC-MS analysis of the fraction giving maximum activity was carried out to know the type of compounds present in the extract. In preliminary screening, fungal isolates obtained were screened for antioxidant activity out of which maximum activity was shown by fungal extract EB i.e 73.6% at 1000μg/ml, total phenolic content and total flavanoid content of the four isolates (GS1, GS3, GR3 and EB) showed that EB has the highest phenolic content i.e 222 mg of QE/g of dried extract and highest flavanoid content of 124 mg of GAE/g of dried extract. EC50 of EB i.e 50% of scavenging activity was observed at a concentration of 350μg/ml and 80% scavenging activity was observed at 1500μg/ml. Qualitative methods such as phytochemical analysis and thin layer chromatography indicated the presence of various compounds. Extraction of TLC fractions yielded four fractions, all the four fractions further gave antioxidant activity out of which third fraction gave the maximum activity 65.3% at 100μg/ml. GC-MS analysis of the third fraction indicated the presence of bioactive compounds 1,2Benzenedicraboxylic acid,diisooctyl ester, 3-tert-butyl-5-chloro-2-hydroxybenzophenone, 4-amino-1,3-diphenyl-quinolin-2-(1H)-one, Octadecane, Phenylseleno-5-methyldihydro- 2(3H)-furanone, Benzaldehyde glycerol acetal and α-tocopherol which may contribute to the extract’s antioxidant activity. Endophytic fungi can thus be a novel source of antioxidant compounds and can play a very important role in food, pharmaceutical and other industries.
|Master of Science- Biotechnology
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