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Title: Sequence Analysis and Biochemical Studies on Catalase (CAT) in Potato (Solanum Tuberosum L.)
Authors: Sharma, Shweta
Supervisor: Das, N.
Keywords: Solanum tuberosum L.;Catalase;Motif search;Enzyme assay;Potato tissues
Issue Date: 18-Sep-2017
Abstract: In plants, supra-optimal light, salinity, insufficient supply of water and mineral nutrients, atmospheric and soil pollutants, attack by microbial pathogens and aerobic metabolic activities are some of the major contributing factors towards stress conditions which lead to the accumulation of reactive oxygen species (ROS). H2O2 is considered to be a potential signal molecule involved in various metabolic processes involved in plant development. Although several type of H2O2 - metabolizing proteins are present in plant systems, catalases are very distinct from other proteins as they catalyze dismutase reaction primarily and have low affinity for H2O2, play an important role in stress-linked H2O2 signaling. Potato is most important non-cereal cash crop ranked fourth after maize in terms of global food production. Plant growth and tuberization are important aspects of potato life cycle which involve generation of stress mainly due to oxidative metabolism. This study focused on some of the commercially important Indian potato cultivars namely Kufri Chipsona-1, Kufri Chipsona-2, Kufri Chandramukhi, Kufri Ashoka, Kufri Pukhraj, and Kufri Jyoti. They represent early, medium and late maturing varieties. The exotic cultivar Desiree was taken as reference. All these plants were grown under field condition, and different tissues like tuber stem and leaves, were collected at different stages of growth. The purpose was to carry out catalase assay in different tissues of the individual cultivars. Tissues were frozen in liquid nitrogen and homogenized in phosphate buffer (pH 7.0). The clarified crude extracts were used for both catalase assay and protein estimation. Catalase activities were found to be significantly different between growing and mature tubers within and between the potato cultivars. The growing tubers showed significantly higher catalase activity as compared to the mature tubers. Leaf tissues showed considerable activities but it was very low in the stem tissues. Efforts were also made for purification of catalase from the tubers and other tissues. In silico analysis was done by retrieving the sequences from the databases. Biochemical attributes, predicting motifs and structures, 3-D modeling and construction of Phylogenetic tree were done and reported in the study. Some of the attributes were not reported earlier. In conclusion, this study on catalase is quite important with regard to the Indian potato cultivars.
Description: Master of Science -Biochemistry
Appears in Collections:Masters Theses@SCBC

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