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Title: Screening and Isolation of α- Amylase Inhibitors from Endophytic Fungi for their Possible use in Management of Type-2 Diabetes
Authors: Dhunna, Avneet
Supervisor: Saxena, Sanjai
Keywords: Vitis vinifer;Endophytic Fungi;Alpha- amylase inhibitors;DNSA;Diabetes
Issue Date: 14-Sep-2017
Abstract: Diabetes mellitus is a chronic, metabolic disorder which is caused either due to insulin deficiency or decrease in insulin in the circulation. Diabetes is characterized by hyperglycaemic condition i.e increase blood sugar levels. In today’s scenario, diabetes has said to affect about 382 million people globally and is responsible for over 5.1 million deaths. According to WHO report, diabetes have high economic burden globally. In 2014, the global economic burden was estimated to be US$612 billion. Endophytic fungi are symbionants residing within the plant. The endophytic fungi are reservoirs of large number of bioactive compounds such as antimicrobial agents, anticancer agents etc. The present study aimed at exploring endophytic fungi isolated from Aegle marmels, Cinnamomum malabaricum, Cinnamum zeylanicum and Vitis vinifera for α-amylase inhibitors. α- amylase inhibitors are compounds which acts on the α-amylase -enzyme responsible for breakdown of carbohydrates inside the body for release of glucose. Thus, these inhibitors are responsible for maintain blood glucose level. The culture filtrates of 105 isolates were obtained and screened for their potential activity against α-amylase inhibitors. The preliminary assay was agar disc diffusion served as both qualitative as well as quantitative method of screening in which 5 isolates showed positive result i.e. #19VVLPM, #13(P)VVLPM, #22(P)VVLPM, #19(P)VVLPM and #107VVLSWN. On basis of percentage of inhibition, isolate culture code #107VVLSWN was selected and subjected to mass production. The compound was extracted by different solvents by liquid –liquid solvent extraction method. The extracted compound was then subjected to qualitative screening by DNSA method. On qualitative screening it was found that DCM fraction exhibited maximum α- amylase inhibitor activity. The different extracted fraction was also subjected for the study of antimicrobial and antioxidant assay. The antimicrobial studies showed that maximum antimicrobial activity was seen in chloroform extract against E.coli. The TLC was also done for the DCM fraction of the isolate #107VVLSWN and 6 bands where observed in the solvent system Hexane: Toluene: Formic acid in ratio of 3:2:.01. On performing morphological studies of the isolate, it was tentatively identified as Alternaria sp. The genomic DNA of the selected isolate was extracted, amplified and further send for sequencing for molecular identification.
Description: Master of Science -Biotechnology
Appears in Collections:Masters Theses@DBT

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