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Title: Isolation and characterization of plant growth promoting rhizobacteria
Authors: Sharma, Divya
Supervisor: Reddy, M. S.
Keywords: PGPR – Plant Growth Promoting Rhizobacteria Phosphorus;Phosphate Solubilizing Bacteria;Nitrogen fixing Bacteria Siderophores
Issue Date: 20-Sep-2013
Abstract: Plant growth-promoting rhizobacteria (PGPR) are the rhizosphere bacteria that can enhance plant growth by a wide variety of mechanisms like phosphate solubilization, siderophore production and biological nitrogen fixation, production of IAA and by HCN production. In the present study PGPRS were isolated from rhizospheric soil and were tested for different plant growth promotion activities. Out of 20 bacterial strains isolated from potato filed, four efficient PGPR strains were selected for further studies. Results of present study showed that these selected strains are efficiently soolubilize the tri calcium phosphate, rock phosphate, aluminium phosphate and ferrous phosphate when suplimented in PKV broth. All the strains are positive for acid, alkaline phosphatase and phytase enzyme production, siderophore production and indole acetic acid production. These selected strains were further tested for their affects on plant growth and soil fertility improvement. When these isolates were used as seed inoculants a significant improvement in plant growth, P uptake and in soil fertility was observed as compared to control soil treatments. The potentiality of PGPR in agriculture is steadily increased as it offers an attractive way to replace the use of chemical fertilizers, pesticides and other supplements. Growth promoting substances are likely to be produced in large quantities by these rhizosphere microorganisms that influence indirectly on the overall morphology of the plants. Use of PGPR as inoculants increased nutrient availability to plants. The progress, to date in using the rhizosphere bacteria in a variety of applications related to agricultural improvement along with their mechanism of action with special reference to plant growth-promoting traits are discussed in this thesis.
Description: Master of Science-Microbiology-Dissertation
Appears in Collections:Masters Theses@DBT

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