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http://hdl.handle.net/10266/2046| Title: | Activity of the Immobilized Candida Rugosa Lipase |
| Authors: | Singh, Kavya |
| Supervisor: | Ali, Amjad |
| Keywords: | Immobilization, Lipase;Candida-Rugosa |
| Issue Date: | 18-Sep-2012 |
| Abstract: | In the present study Candida rugosa lipase has been immobilized on MCM- 41 support through sol – gel process. The immobilized enzyme was entrapped by the poly condensation of silica precursors namely tetramethoxyorthosilane /tetraethoxyorthosilane(TEOS) and iso-butyltrimethoxysilane (iso- BTMS). The immobilized enzyme was characterized by scanning electron microscopy, Fourier Transformed Infrared (FT–IR) and UV–Visible Spectrophotometer. Kinetic parameters viz., Michaelis-Menten constant (Km), Maximum rate of reaction (Vmax), Turnover number (kcat) and Specificity constant (kcat /Km) for immobilized Candida rugosa lipase were determined by using Michaelis-Menten and Lineweaver- Burk approach. |
| Description: | Master of Science (Chemistry) |
| URI: | http://hdl.handle.net/10266/2046 |
| Appears in Collections: | Masters Theses@SCBC |
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