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Title: Activity of the Immobilized Candida Rugosa Lipase
Authors: Singh, Kavya
Supervisor: Ali, Amjad
Keywords: Immobilization, Lipase;Candida-Rugosa
Issue Date: 18-Sep-2012
Abstract: In the present study Candida rugosa lipase has been immobilized on MCM- 41 support through sol – gel process. The immobilized enzyme was entrapped by the poly condensation of silica precursors namely tetramethoxyorthosilane /tetraethoxyorthosilane(TEOS) and iso-butyltrimethoxysilane (iso- BTMS). The immobilized enzyme was characterized by scanning electron microscopy, Fourier Transformed Infrared (FT–IR) and UV–Visible Spectrophotometer. Kinetic parameters viz., Michaelis-Menten constant (Km), Maximum rate of reaction (Vmax), Turnover number (kcat) and Specificity constant (kcat /Km) for immobilized Candida rugosa lipase were determined by using Michaelis-Menten and Lineweaver- Burk approach.
Description: Master of Science (Chemistry)
Appears in Collections:Masters Theses@SCBC

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