Please use this identifier to cite or link to this item: http://hdl.handle.net/10266/4861
Title: Isolation and Purification of Xanthine Oxidase Inhibitor from Endophytic Fungi
Authors: Agarwal, Ankita
Chhibber, Manmohan (Guide)
Saxena, Sanjay (Guide)
Keywords: Allopurinol
Febuxostat
Uric acid
Hypoxanthine
Nitroblue-tetrazolium assay
Ninhydrin test
Issue Date: 13-Sep-2017
Abstract: Endophytic fungi are a treasure of bioactive compounds with therapeutic properties due to their symbiotic association with host organisms. This research aims at isolating xanthine oxidase inhibitors (XOIs) for the treatment of gout from endophytic fungi. Extract of the fungal strains, isolated from Camellia sinensis, grown on PDA exhibited appreciable inhibitory activity against xanthine oxidase. The extract was purified using column chromatography to isolate five pure fractions. Two of the five pure fractions (Fraction -2 and Fraction - 4) gave best results when evaluated using NBT assay. Phytochemical testing was also carried out to identify secondary metabolites present. All the pure fractions obtained were also subjected to antioxidant assay and evaluated for their antibacterial activity. While antioxidant assay was done using DPPH radical scavenging activity, antimicrobial activity was carried out against various bacterial cultures using disk diffusion method on MHA plates. Genomic DNA of the fungus was also isolated and amplified using PCR and its products.
Description: Master of Science -Chemistry
URI: http://hdl.handle.net/10266/4861
Appears in Collections:Masters Theses@SCBC

Files in This Item:
File Description SizeFormat 
301502004 Thesis.pdf16.49 MBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.